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Fig. 2 Direct ex vivo cross-recognition analysis of disease-relevant islet-specific T cells. Direct ex vivo cross-recognition analysis of disease-relevant.

Published byLiana Pranoto Modified over 5 years ago

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Presentation on theme: "Fig. 2 Direct ex vivo cross-recognition analysis of disease-relevant islet-specific T cells. Direct ex vivo cross-recognition analysis of disease-relevant."— Presentation transcript:

1 Fig. 2 Direct ex vivo cross-recognition analysis of disease-relevant islet-specific T cells.
Direct ex vivo cross-recognition analysis of disease-relevant islet-specific T cells. (A) Double staining by DQ8cis and DQ8trans tetramers was observed for ex vivo polyclonal GAD65250–266-specific cells in DQ8+DQ2− participants. DQ8trans/HA102–188 tetramer was used as a control. The double staining shown here was performed in DQ8 (DQA1*0301-DQB1*0302) homozygous individuals. (B) Ex vivo sorted DQ8trans/IA-2957–972–specific clones cross-recognized DQ8cis and DQ8trans. (C and D) Comparison of total (left) and memory (right) CD4+ T cell frequencies for GAD65250–266-specific (C) and for IA-2957–972–specific (D) T cells among DQ8+DQ2− patients (closed circles), DQ8+DQ2+ patients (open circles), and DQ8+ healthy controls (gray circles). The y axis in (C) and (D) indicates T cell frequencies. Statistical significance was evaluated by one-way analysis of variance (ANOVA) followed by Bonferroni’s test. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant (P > 0.05). Tmr, tetramer. I-Ting Chow et al. Sci Adv 2019;5:eaaw9336 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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