1. Epicutaneous Sensitization with Protein Antigen Induces Th9 Cells
Jing-Yi Lin, Jau-Shiuh Chen, Chih-Jung Hsu, Shi-Chuen Miaw, Ching-Yi Liu, Shu-Jen Lee, Pei-Chun Chen, Li-Fang Wang 
Journal of Investigative Dermatology 
Volume 132, Issue 3, Pages (March 2012)
DOI: /jid
Copyright © 2012 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Induction of T helper type 9 (Th9) cells by epicutaneous sensitization with ovalbumin (OVA). Groups of BALB/c (n=5) (a) or DO11.10 (n=5; a–c) mice were sensitized by patch application with OVA solution or phosphate-buffered saline (PBS) control. After 10 days, draining lymph nodes were harvested. (a) The IL-9 contents of supernatants of in vitro reactivation cultures of the pooled draining lymph node cells were determined by ELISA. (b, c) CD4 T cells were purified after in vitro OVA reactivation cultures for 24hours with the addition of monesin for the last 4hours. In addition, intracellular staining for various cytokines was performed and cells were analyzed by flow cytometry. APC, allophycocyanin; PE, phycoerythrin.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2012 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
IL-9 promotes the T helper type 2 (Th2) response induced by epicutaneous sensitization with ovalbumin (OVA). Groups of BALB/c mice (n=5) received phosphate-buffered saline (PBS) or OVA patch application on days 1–5 along with (a) intraperitoneal (i.p.) IL-9 or PBS on days 0, 4, and 8 and (b) i.p. anti-IL-9 antibody or isotype control on day 0. After 10 days, the IL-5 and IL-13 contents of supernatants of in vitro OVA reactivation culture were determined. (c) Groups of BALB/c mice (n=10) received another OVA challenge course on days 22–26. On day 29, blood was obtained and serum levels of OVA-specific IgE, IgG1, and IgG2a were determined by ELISA. T-test with unequal variance was adopted to test the difference between two groups; the P-values for IgE, IgG1, and IgG2a are 0.014, 0.015, and 0.440, respectively. OD, optical density.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2012 The Society for Investigative Dermatology, Inc Terms and Conditions