1. Chromatographic Techniques
Day 1
9:00 Introduction
9:15 Lecture: Gel filtration
10:00 Coffee Break
10:15 Introduction practical work and AKTA System demo
11:00 Exp 1: Gel Filtration-Desalting mode
11:30 Lecture: Ion Exchange
12:30 Lunch
1:30 Discussion results Exp.1/ Introduction Exp. 2
2:00 Practical: Exp2: Ion exchange- Media/pH scouting
2:30 Lecture: Analytical Techniques
3:30 Coffee Break
4:00 Discussion of results Exp. 2/ Intro Exp. 3
4:30 Practical: Exp.3: Ion exchange- binding capacity
6:00 End of day 1

2. Chromatographic Techniques
Day 2
8:30 Discussion of results Exp. 3/ Intro Exp. 4
8:45 Practical: Exp.4: IEC scouting for step 2
9:30 Lecture: Purification Strategies
10:15 Coffee break
10:30 Analysis: Discussions results Exp.4
11:00 Lecture: Hydrophobic interactions &
Reversed phase chromatography
12:00 Lunch
1:00 Intro Exp. 5
1:15 Practical: Exp.5: HIC-scouting
2:00 Lecture: Affinity chromatography
2:45 Analysis: Discussions results Exp. 5/ Intro Exp.6
3:15 Practical: Exp.6: IEC/ HIC contd. (step 2)
3:30 Coffee break
3:45 Lecture: Optimization (general)
5:00 End of day

3. Chromatographic Techniques
Day 3
8:30 Lecture: Optimization (different techniques
9:30 Discussion of results Exp. 6/ Intro Exp. 7
9:45 Practical: Exp. 7: Affinity chromatography
10:15 Coffee break
10:30 Lecture: Scale up and fine tuning
11:30 Discussion of results Exp. 7
12:00 Lunch
1:00 Lecture: Process hygiene
1:45 Lecture: Regulation: A brief introduction
2:15 Coffee Break
2:30 Lecture: Column Packing (Video)
3:00 Discussion of results and Q&A
4:00 End of Course