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Fig. 4 Experimental design for algesimetry.

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Presentation on theme: "Fig. 4 Experimental design for algesimetry."— Presentation transcript:

1 Fig. 4 Experimental design for algesimetry.
Experimental design for algesimetry. The antinociceptive effect of NPs was tested in a pathophysiological context induced by an intraplantar carrageenan injection (2% saline, 100 μl). Involvement of central or peripheral opioid receptors was performed using a brain-permeant opioid antagonist, naloxone (Nal), and a brain-impermeant opioid receptor antagonist, naloxone methiodide (Nal-M). NP suspensions or control solutions were injected intravenously with a dose volume of 10 ml/kg during 30 s. The Hargreaves test was performed 10 min after the NP administration and then every 30 min up to a period of 250 min. The dose of LENK-SQ NPs (20 mg/kg) was equivalent to LENK (11.48 mg/kg) and to SQ NPs (8.28 mg/kg) and corresponded to mmol/kg for both LENK-SQ and LENK. s.c., subcutaneous; i.pl., intraplantar. Jiao Feng et al. Sci Adv 2019;5:eaau5148 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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