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Triple sugar iron (TSI) slant
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Triple sugar iron (TSI) slant
Albert Tsai, MD, PhD
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Triple sugar iron (TSI) slant
1 glucose 10 lactose 10 sucrose acid pH indicator yellow O2 pH indicator peptone alkaline red ferric ammonium citrate + H2S black Albert Tsai, MD, PhD
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Triple sugar iron (TSI) slant
ferments lactose/sucrose produces H2S ferments glucose A/A E. coli Klebsiella Enterobacter Yersinia Serratia A/A+H2S Proteus runs out of glucose, cannot use lactose / sucrose, uses peptone cannot ferment, uses peptone produces H2S K/K, K/NC Pseudomonas Acinetobacter Eikenella Moraxella K/A Shigella Morganella Providencia K/A+H2S Salmonella Edwardsiella Albert Tsai, MD, PhD
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Triple sugar iron (TSI) slant
ferments lactose/sucrose produces H2S ferments glucose A/A E. coli Klebsiella Enterobacter Yersinia Serratia A/A+H2S Proteus runs out of glucose, cannot use lactose / sucrose, uses peptone cannot ferment, uses peptone produces H2S K/K, K/NC Pseudomonas Acinetobacter Eikenella Moraxella K/A Shigella Morganella Providencia K/A+H2S Salmonella Edwardsiella Albert Tsai, MD, PhD
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N,N-dimethyl-p-phenylenediamine
Oxidase test cytochrome oxidase + + + 4 4 3 O2 3 H2O N,N-dimethyl-p-phenylenediamine α naphthol – indophenol blue and
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– + Catalase test + catalase 2 H2O2 2 H2O O2 hydrogen peroxide water
oxygen –
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– + Indole test + + + tryptophanase tryptophan water indole pyruvate
ammonium Kovac reagent para-dimethylaminobenzaldehyde, isoamyl alcohol, concentrated HCl – red Erlich reagent p-dimethylaminocinnamaldehyde blue-green and
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Nitrate reductase test
nitrite reductase NO3- NO2- NH3 or N2 nitrate nitrite ammonia nitrogen α napthylamine, sulfanilic acid α napthylamine, sulfanilic acid α napthylamine, sulfanilic acid no change red no change zinc ? – zinc NO2- no change nitrite α napthylamine, sulfanilic acid red
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Ornithine decarboxylase test ornithine decarboxylase
+ ornithine putrescine carbon dioxide – pH indicator purple (alkaline) glucose fermentation yellow (acid) pH indicator and
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– + Urease test + + urease H2O 2 NH3 CO2 carbon dioxide urea water
ammonia phenol red – fuschia (alkaline) and
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