1. Human cumulus cell gene expression as a biomarker of pregnancy outcome after single embryo transfer 
Kathryn Michelle Gebhardt, Ph.D., Deanne Kate Feil, Ph.D., Kylie Renee Dunning, Ph.D., Michelle Lane, Ph.D., Darryl Lyndon Russell, Ph.D. 
Fertility and Sterility 
Volume 96, Issue 1, Pages e2 (July 2011)
DOI: /j.fertnstert
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2. Figure 1
Cumulus cell gene expression associated with pregnancy and live birth. Quantitative real-time RT-PCR analysis of mRNA expression (mRNA copy number relative to GAPDH) of (A) VERSICAN, (B) PTGS2, and (C) PTX3 in human cumulus cells from oocytes that produced a live birth versus those for which pregnancy was not established after SET. ∗Significant difference (P=.02); #Trend (P=.06).
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3. Figure 2
Cumulus cell gene expression not associated with pregnancy. Quantitative real-time RT-PCR analysis of mRNA expression (mRNA copy number relative to GAPDH) in human cumulus cells from oocytes that produced a live birth versus those for which pregnancy was not established after SET. (A) metabolic enzymes in the glycolytic pathway ALDOA, PFKP, LDHA, and PKM2. (B) Cell-signaling mediators STS, GREM1, and AHR. (C) Extracellular matrix genes HAS2 and TNFAIP6. No significant differences were observed.
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4. Figure 3
Cumulus cell gene expression correlating with birth weight. A Spearman’s rank order correlation of birth weight (grams) and the mRNA abundance (copy number relative to GAPDH) of (A) VCAN, (B) GREM1, and (C) PFKP in cumulus cells from oocytes that produced a pregnancy after SET. VCAN and GREM showed a trend toward significance (P≤.08), while cumulus cell PFKP expression was significantly (P<.05) correlated with birth weight.
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5. Supplementary Figure 1
Cumulus cell gene expression was not associated with embryo morphological grade. mRNA expression (copy number relative to GAPDH) in matched pairs (n = 11 pairs) of human cumulus cells from oocytes that developed to high-grade versus low-grade embryos. (A) PTGS2, (B) VERSICAN, (C) PTX3, (D) TNFAIP6, (E) HAS2, (F) GREM1, (G) PFKP, and (H) LDHA. No significant differences were observed.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
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