1. STAT5 as a molecular regulator of proliferation, differentiation and apoptosis in hematopoietic cells
IL‐3‐induced apoptosis in Ba/F3 cells expressing the 1*6 mutant STAT5A‐Flag. (A) DNA ladder formation in Ba/F3 cells expressing the 1*6 mutant STAT5A after IL‐3 treatment. The cells expressing the wild‐type STAT5A‐Flag was deprived of IL‐3 for 40.5 h and those expressing the 1*6 mutant STAT5A‐Flag were treated with 3 ng/ml of IL‐3 for 24 h. −, no IL‐3; +, culture with IL‐3; Marker, DNA ladder marker (Bio‐Rad, 50, 100, 200, 300, 400, 500, 700, 1000, 1500, 2000 bp). (B) The rate of apoptotic events in Ba/F3 cells expressing the mutant STAT5A‐Flag after stimulation with IL‐3. Bulk culture of the stable transfectant expressing the 1*6 mutant STAT5A‐Flag was treated with 3 ng/ml of IL‐3, and morphologically apoptotic cells were enumerated by trypan blue exclusion test in triplicate.
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EMBO J, Volume: 18, Issue: 17, Pages: , First published: 01 September 1999, DOI: ( /emboj/ )