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Extracellular matrix and type I collagen promote a scattered phenotype and induce cell motility.
Extracellular matrix and type I collagen promote a scattered phenotype and induce cell motility. Extracellular matrices deposited by HPDE cells, Panc1 cells, and pancreatic F5 fibroblasts were generated as described in Materials and Methods. A, matrices were then extracted using Laemmli sample dilution buffer and analyzed for type I collagen, laminin 1, and laminin 5 by Western blot analysis. B, HPDE cells were plated for 24 hours onto its own matrix, Panc1 matrix, pancreatic F5 fibroblast matrix, or type I collagen, and phase-contrast pictures were taken using Nikon camera. C, colloidal gold phagokinetic tracks generated by HPDE cells (103) plated for 18 hours onto its own matrix, Panc1 matrix, pancreatic F5 fibroblast matrix, or type I collagen were visualized using dark-field illumination. D, the area generated by the motile cells was quantified, and the average area of individual tracks (including circular clearings) was compared with the area of the tracks and clearings generated by HPDE cells. *, P < 0.05, significantly different from control HPDE matrix. Representative of three independent experiments. Adam J. Ottaviano et al. Cancer Res 2006;66: ©2006 by American Association for Cancer Research
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