1. Volume 7, Issue 7, Pages 1191-1210 (July 2014)
Spatial H2O2 Signaling Specificity: H2O2 from Chloroplasts and Peroxisomes Modulates the Plant Transcriptome Differentially 
Sewelam Nasser , Jaspert Nils , Van Der Kelen Katrien , Tognetti Vanesa B. , Schmitz Jessica , Frerigmann Henning , Stahl Elia , Zeier Jürgen , Van Breusegem Frank , Maurino Veronica G.  
Molecular Plant 
Volume 7, Issue 7, Pages (July 2014)
DOI: /mp/ssu070
Copyright © 2014 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
H2O2 Accumulation and Photoinhibition in GO5 and cat2-2 Plants.
(A) DAB staining showing H2O2 production in wild-type (Col-0), GO5 and cat2-2 after transfer of high-CO2-grown plants to normal air at 75 μmol quanta m–2 s–1.
(B) Maximum quantum yield of PSII (FV/FM) determined in Col-0, GO5, and cat2-2 at different time points after transfer of high-CO2-grown plants to normal air and of the same plants maintained in high-CO2 conditions.
Molecular Plant 2014 7, DOI: ( /mp/ssu070)
Copyright © 2014 The Authors. All rights reserved. Terms and Conditions

3. Figure 2
Comparative Metabolic Profile of Col-0, GO5 and cat2-2 Plants.
(A) Heat map of individual metabolites comparing ambient CO2 and high CO2 in wild-type (Col-0), GO5, and cat2-2 plants (log2-fold change).
(B) PCA score plot of GC/MS metabolite data.
(C) PCA loadings plot of GC–EI–TOF–MS metabolite data.
Molecular Plant 2014 7, DOI: ( /mp/ssu070)
Copyright © 2014 The Authors. All rights reserved. Terms and Conditions

4. Figure 3
Representations of the Number of Induced Genes in GO5 and cat2-2 Plants and Their Classification in Different Functional Categories.
(A) Venn diagram of the number of H2O2-responsive genes up-regulated only in GO5 or cat2-2 and in both, GO5 and cat2-2, after 8h of H2O2 production.
(B) Pie chart of the functional categories of genes whose expression was up-regulated specifically by chloroplastic-produced H2O2.
(C) Pie chart of the functional categories of genes whose expression was up-regulated by both chloroplastic- and peroxisomal-produced H2O2.
(D) Pie chart of the functional categories of genes whose expression was up-regulated specifically by peroxisomal-produced H2O2.
Molecular Plant 2014 7, DOI: ( /mp/ssu070)
Copyright © 2014 The Authors. All rights reserved. Terms and Conditions

5. Figure 4
Dynamics of the Transcriptional Responses of Selected Genes in GO5 and cat2-2 Plants Analyzed by qRT–PCR.
The asterisk (*) indicates statistically significant difference calculated by the two-tailed Student’s t-test (P < 0.05) to the time point t = 0.
Molecular Plant 2014 7, DOI: ( /mp/ssu070)
Copyright © 2014 The Authors. All rights reserved. Terms and Conditions

6. Figure 5
Schematic Representation of the Camalexin and Indolic Glucosinolate Biosynthetic Pathways Showing the Genes Up-Regulated by Chloroplastic-Produced H2O2.
FC indicates the fold-change induction of gene expression with respect to the wild-type. I3M = indol-3-ylmethyl glucosinolate = Glucobrassicin; 4OH-I3M = 4-hydroxy-indol-3-ylmethyl glucosinolate = 4-hydroxyglucobrassicin; 4MO-I3M = 4-methoxy-indol-3-ylmethyl glucosinolate = 4-methoxyglucobrassicin.
Molecular Plant 2014 7, DOI: ( /mp/ssu070)
Copyright © 2014 The Authors. All rights reserved. Terms and Conditions

7. Figure 6
Diagram Showing the Spatial H2O2 Signaling Effects Occurring in the GO5 and cat2-2 Plants after 8h of Moderate Production of H2O2.
H2O2 originated in chloroplasts or peroxisomes can induce two different kinds of responses: one which is dependent on the subcellular production site and the other which integrates H2O2 signals independently from the subcellular site of its production.
Molecular Plant 2014 7, DOI: ( /mp/ssu070)
Copyright © 2014 The Authors. All rights reserved. Terms and Conditions