1. Summary of branch point mapping in P. falciparum.
Summary of branch point mapping in P. falciparum. (A) Schematic of the RT-PCR approach to map the branch points in four P. falciparum introns. (B) Agarose gel of PCR products amplified from RNA lariats of receptor for activated C kinase homolog (PfRACK, PF08_0019). + or − indicates whether the RNA sample was treated with RNase R prior to reverse transcription. M, DNA marker; mR, mRNA; lar, lariat. DNA marker sizes in base pairs are on the left. (C) The experimentally determined branch points are indicated by underlined letters. The distance of each branch point from the 3′ splice site is indicated. (D) Summary of branch point information.
Xiaohong Zhang et al. Eukaryotic Cell 2011; doi: /EC