1. Drug sensitivities of IGROV1 cells to AGF94 and PMX at leucovorin (LCV) concentrations of 2 and 25 nmol/L.
Drug sensitivities of IGROV1 cells to AGF94 and PMX at leucovorin (LCV) concentrations of 2 and 25 nmol/L. Cells were plated (4,000 cells/well) in folate-free RPMI1640 medium with 10% dialyzed serum, antibiotics, l-glutamine, and 2 or 25 nmol/L LCV with a range of concentrations of AGF94 or PMX. Cell proliferation was assayed with CellTiter-Blue and a fluorescent plate reader. Results for drug treatments were normalized to relative growth in the absence of drug additions. Results are shown as mean values ± standard errors (error bars) from six separate experiments. IC50s for AGF94 were 0.39 ± 0.06 nmol/L and 4.14 ± 0.94 nmol/L at 2 and 25 nmol/L LCV, respectively. IC50s for PMX were 57.7 ± 3.0 nmol/L and 346 ± 26 nmol/L at 2 and 25 nmol/L LCV, respectively.
Zhanjun Hou et al. Mol Cancer Ther 2017;16:
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