1. Ifnar1+/− placentas show increased apoptosis and abnormal maternal-fetal blood barrier.
Ifnar1+/− placentas show increased apoptosis and abnormal maternal-fetal blood barrier. Ifnar1−/− females mated to Ifnar1+/− males were infected intravaginally with 1.5 × 105 PFU Cambodian ZIKV at E5.5 and harvested at E10.5. Paraffin-embedded sections were stained for Ki67 (A, dividing cells) or cleaved Casp3 (B, apoptotic cells) with DAB. From (A) and (B), images from labyrinth are shown. Scale bars, 50 μm. Representative images from at least three placentas/deciduas per condition from at least two litters are shown. (C) Placentas were fixed at least 24 hours in formaldehyde with 2.5% glutaraldehyde at 4°C. The labyrinth was dissected and processed for electron microscopy. Uninfected Ifnar1+/− labyrinth shows four layers of cells between frbc and mrbc: fetal endothelial cell (ec), two syncytiotrophoblast layers (ST-II, ST-I), and the sinusoidal trophoblast giant cell (stgc). Infected Ifnar1−/− placenta is similar to the uninfected control. The barrier between maternal and fetal blood of ZIKV-infected Ifnar1+/− placentas is highly abnormal with unfused cells (left). ZIKV-infected Ifnar1+/− shows multiple examples of mixing between maternal and fetal blood (right). Scale bars, 2 or 10 μm as labeled on the image. Multiple sections and planes from one placenta per condition were analyzed.
Laura J. Yockey et al. Sci. Immunol. 2018;3:eaao1680
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