1. Increased levels of inflammation in mo-DCs translate into higher numbers of specific CD8 T-cell responses.
Increased levels of inflammation in mo-DCs translate into higher numbers of specific CD8 T-cell responses. Healthy donor naïve A2.1+ CD8+ T cells were primed with peptide encoding for the HLA A2.1 restricted Mart-1/MelanA (ELAGIGILTV) epitope and the frequency of tetramer positive cells was monitored (n = 6; A). Tetramer positive cells from all conditions from a donor were expanded and examined functionally for secretion of IL-13, IL-5, IFNγ, TNFα, and IL-2 (B, C, D, E, and F, respectively). Differentially matured mo-DCs and allogeneic naïve CD8+ T cells were cocultured for 6 days in a MLR reaction in presence of vehicle (−), rhIL-12, anti-IL-12, or IgG. Proliferated cells were stimulated overnight with αCD3/CD28 beads and evaluated for the production of IFN-γ. **, P < 0.01 when comparing PolyI:C to LPS (with vehicle) and when comparing PolyI:C (with vehicle) to PolyI:C when IL-12 was blocked. NS, not significant when comparing PolyI:C (with vehicle) to PolyI:C when IgG was used (G). Healthy donor naïve A2.1+ CD8+ T cells were primed with peptide encoding for the HLA A2.1 restricted Mart-1/MelanA (ELAGIGILTV) epitope and the frequency of tetramer positive cells was monitored in 2 additional donors with a condition of αIL-10/IL10R Abs for each treatment, showing a representative donor (triplicate wells; H).
Dusan Bogunovic et al. Cancer Res 2011;71:
©2011 by American Association for Cancer Research