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Artemisia santolinaefolia Artemisia scoparia

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Presentation on theme: "Artemisia santolinaefolia Artemisia scoparia"— Presentation transcript:

1 Artemisia santolinaefolia Artemisia scoparia
Supplemental material 1 Pictures of plants Artemisia santolinaefolia Artemisia scoparia

2 A B Supplemental material 2
Supplemental material 2. Diet-induced obese mice were treated with or without SANT and SCO for 4 weeks. Body weight and food intake were recorded weekly. (A) body weight results and (B) food intake data in three groups of animals. There are no significant differences between these parameters.

3 HMW MMW LMW Supplemental material 3
Non-denatured plasma adiponectin levels in mice HMW MMW LMW Mouse # Group HFD SANT SCO Supplemental material 3. High molecular weight (HMW) species, medium molecular weight (MMW) form of the complexes, and low molecular weight form (LMW) adiponectin in the mice. Plasma adiponectin levels were determined by western blotting assay using non-denatured plasma. Mean ± SEM (n=5), * P<0.05 and ** P<0.01 SANT or SCO vs. HFD.

4 A B Supplemental material 4 Adiponectin
Adiponectin content in the media of 3T3 adipocytes Adiponectin Dose (g/ml) Extract Control SANT SCO Supplemental material 4. Effects of SANT and SCO on adiponectin secretion and its gene expression in 3T3 adipocytes. 3T3 adipocytes were cultured in 12-well plates, then were treated with or without various doses of SANT and SCO overnight (14 h), media were collected and adiponectin levels in the media were determined using a western blotting assay. the effects of dose-response of SANT and SCO on media adiponectin content in 3T3 adipocytes. (B) Gene expression of adiponectin was measured using a quantitative real time PCR assay in 3T3 adipocytes treated with or without 25 g/ml SANT or 25 g/ml SCO overnight. Date are mean ± SEM from three separated experiments. *P<0.05 and *** P<0.001 SANT or SCO vs. Control.

5 C2C12 myotubes Supplemental material 5 IRS-1 IRS-2 PGC-1 AMPK p
ACC p ACC β-actin Extract C SANT SCO PMI SANT SCO PMI Dose µg/ml g/ml Supplemental material 5. Extracts of Santa, SCO and PMI-5011 (PMI) on PGC-1α, insulin and AMPK signaling proteins in L6 myotubes. L6 myotubes were treated with extracts at doses of 5 g/ml and 10 g/ml for 14 hrs. Cell lysates were subjected to SDS-PAGE, and transferred to nitrocellulose membranes. IRS-2, PGC-1α and AMPK signaling protein abundance was measured by western blotting.


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