1. Effect of TLR-4 ligation with LPS on NF-κB activation in EOC cells: (A) MyD88+ and (B) MyD88− EOC cells were treated with 10 μg/mL LPS for 1, 2, and 4 hours, and the nuclear and cytoplasmic fractions were separated as described in Materials and Methods.
Effect of TLR-4 ligation with LPS on NF-κB activation in EOC cells: (A) MyD88+ and (B) MyD88− EOC cells were treated with 10 μg/mL LPS for 1, 2, and 4 hours, and the nuclear and cytoplasmic fractions were separated as described in Materials and Methods. The translocation of p65 from the cytoplasm into the nucleus was determined by Western blot analyses. Topoisomerase was included to show integrity of the fractions. C, levels of IκB-α in MyD88+ and MyD88− EOC cells following LPS stimulation. Note the decrease in IκB expression in MyD88+ cells but not in MyD88− EOC cells. Results from R182 (MyD88+) and A2780 (MyD88−). Similar results were obtained from other EOC cell lines.
Michael G. Kelly et al. Cancer Res 2006;66:
©2006 by American Association for Cancer Research