1. MLN944 intercalates into DNA
MLN944 intercalates into DNA. Intercalation was monitored by conversion of relaxed plasmid DNA to supercoiled molecules (SC).
MLN944 intercalates into DNA. Intercalation was monitored by conversion of relaxed plasmid DNA to supercoiled molecules (SC). An agarose gel stained with ethidium bromide is shown. Control reactions were carried out in the absence of enzyme [(−) SC, Relaxed] or drug (No Drug). A MLN944 titration (0.1–5.0 μm) was carried out with constant topoisomerase I concentration. Reactions containing etoposide (100 μm) and ethidium bromide (10 μm) are included as examples of a nonintercalative and intercalative drug, respectively.
Darshan S. Sappal et al. Mol Cancer Ther 2004;3:47-58
©2004 by American Association for Cancer Research