1. * Supplementary Fig. 1 (Yamanaka et al.) * * * ** ** ** * * **
mca1
mca2
Wild type
MCA1ox
mca1 mca2
MCA2ox-1
MCA2ox-2
MCA2ox-3 * ** ** ** * *
Wild type
mca1
mca2
MCA1ox
mca1 mca2
MCA2ox-1
MCA2ox-2
MCA2ox-3 **
Wild type
mca1
mca2
MCA1ox
mca1 mca2
MCA2ox-1
MCA2ox-2
MCA2ox-3
Supplementary Fig. 1　 The levels of the MCA1 and MCA2 transcripts in various MCA1 and MCA2 mutants. Total RNA was purified from five to ten plants grown for 13 days on MS medium and transcript levels of the MCA1 and MCA2 genes were measured by quantitative real-time PCR as described in Materials and Methods in the text. Expression of the EF1a gene was also examined as a control. The transcript levels of each gene were compensated with those of b-tubulin as an internal standard. The transcript levels in mutants are presented relative to those in the wild type, which have been assigned a value of 1. Values are the mean + SD for three independent experiments. MCA2ox-1, MCA2ox-2, and MCA2ox-3 represent three independent MCA2-overexpression lines. *p < 0.005; **p < 0.05.