1. LY2780301 induces cytotoxicity in hMECs via inhibition of Akt/p70S6K kinase activities.
LY induces cytotoxicity in hMECs via inhibition of Akt/p70S6K kinase activities. A, Cell growth curve of 10A.Vec and 10A.B2 cells treated with LY DMSO was used as vehicle control. B, Western blotting of PCNA in 10A.Vec and 10A.B2 cells treated with LY at various dosages. Quantification of PCNA band was shown underneath each lane. C, Flow cytometry dot plot of 10A.Vec and 10A.B2 cells treated with LY and stained with PI and BrdU-APC for cell-cycle determination. Quantification of each subgroup (G0–G1 phase, S-phase, and G2–M phase) under different treatment conditions was summarized and shown to the right of the plot.
Xiao Wang et al. Cancer Prev Res 2017;10:
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