1. The Escherichia coli RNA polymerase α subunit linker: length requirements for transcription activation at CRP‐dependent promoters
Comparison of the effect of deletions and insertions within the α inter‐domain linker on transcription from CRP‐dependent promoters in vitro and in vivo. (A) Amount of transcripts produced by RNAP reconstituted with each mutant α subunit in the presence of CRP at the CC(−41.5) (▴) and CC(−61.5) (○) promoters in vitro. Data are taken from Figure 3. (B) Activity of the CC(−41.5) (▴) and CC(−61.5) (○) promoters in cells expressing chromosome‐encoded wild‐type α and plasmid‐encoded mutant or wild‐type α. Data are taken from Figure 8. Values in both panels are expressed as a percentage of the promoter activity in the presence of RNAP containing only wild‐type α.
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EMBO J, Volume: 19, Issue: 7, Pages: , First published: 03 April 2000, DOI: ( /emboj/ )