1. Changes of gene expression induced by 1 mmol/L valproic acid in vitro.
Changes of gene expression induced by 1 mmol/L valproic acid in vitro. Quantitative real-time PCR was used to examine the temporal changes of mRNA transcripts during valproic acid treatment (up to 28 d) using normal cDNAs from two adult cerebellum (Adult), two age-matched pediatric cerebella (Child), and one fetal brain (Fetal) as references (A). The concomitant changes of protein expression were analyzed with Western hybridization (B). Coomassie blue–stained proteins on polyacrylamide gel were used as loading control. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Xiao-Nan Li et al. Mol Cancer Ther 2005;4:
©2005 by American Association for Cancer Research