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IgE sensitization profiles toward green and gold kiwifruits differ among patients allergic to kiwifruit from 3 European countries  Merima Bublin, MSc,

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Presentation on theme: "IgE sensitization profiles toward green and gold kiwifruits differ among patients allergic to kiwifruit from 3 European countries  Merima Bublin, MSc,"— Presentation transcript:

1 IgE sensitization profiles toward green and gold kiwifruits differ among patients allergic to kiwifruit from 3 European countries  Merima Bublin, MSc, Adriano Mari, MD, Christof Ebner, MD, André Knulst, MD, Otto Scheiner, PhD, Karin Hoffmann-Sommergruber, PhD, Heimo Breiteneder, PhD, Christian Radauer, PhD  Journal of Allergy and Clinical Immunology  Volume 114, Issue 5, Pages (November 2004) DOI: /j.jaci Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 Immunoblot analysis of kiwifruit protein extracts. Green and gold kiwifruit extracts were blotted to polyvinylidene difluoride membranes: (1) Coomassie stain, (2) IgE immunoblot with a pool of kiwifruit allergic patients' sera, (3) IgE immunoblot with a pool of sera from nonallergic donors, (4) immunoblot with rabbit antigrape chitinase, (5) immunoblot with rabbit antigrape thaumatin, and (6) buffer as a negative control for rabbit antibodies. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 A, Symptoms of patients and specific IgE against kiwifruit extracts and actinidin (A, asthma; AD, atopic dermatitis; D, dyspnea; OAS, oral allergy syndrome; U, urticaria). B, ELISA inhibition analysis of IgE binding to kiwifruit extracts by using green kiwifruit extract (black bars), gold kiwifruit extract (white bars), and actinidin (gray bars) as inhibitors. Inhibitors were used at concentrations of 4, 20, and 100 μg/mL. ND, Not done. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 IgE Immunoblot with 9 Austrian and 11 Italian sera showing IgE binding to green and gold kiwifruit protein extracts. B, Buffer control; N, serum pool of nonallergic subjects. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

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