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Farmer's lung: Patients’ IgG2 antibodies specifically recognize Saccharopolyspora rectivirgula proteins and carbohydrate structures  Cornelia Mundt, PhD,

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Presentation on theme: "Farmer's lung: Patients’ IgG2 antibodies specifically recognize Saccharopolyspora rectivirgula proteins and carbohydrate structures  Cornelia Mundt, PhD,"— Presentation transcript:

1 Farmer's lung: Patients’ IgG2 antibodies specifically recognize Saccharopolyspora rectivirgula proteins and carbohydrate structures  Cornelia Mundt, PhD, Wolf-Meinhard Becker, PhD, Max Schlaak, MD  Journal of Allergy and Clinical Immunology  Volume 98, Issue 2, Pages (August 1996) DOI: /S (96) Copyright © 1996 Mosby, Inc. Terms and Conditions

2 FIG. 1 IEF of total S. rectivirgula extract in a Rotofor cell. Electrophoresis was performed with a pH gradient of 3 to 10. A, Coomassie brilliant blue (Serva, Heidelberg, Germany) staining of extract. B, IgG2 binding of patients’ pooled sera to the total extract. Lanes 1 to 17, Rotofor fractions. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

3 FIG. 1 IEF of total S. rectivirgula extract in a Rotofor cell. Electrophoresis was performed with a pH gradient of 3 to 10. A, Coomassie brilliant blue (Serva, Heidelberg, Germany) staining of extract. B, IgG2 binding of patients’ pooled sera to the total extract. Lanes 1 to 17, Rotofor fractions. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

4 FIG. 2 Western blot of S. rectivirgula extract binding to patients’ IgG2. I, India ink staining; FL, patients’ IgG2 binding (n = 22); E, IgG2 reactivity with an exposed person’s pooled serum; K, antibody control; kDa, MW in kilodaltons. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

5 FIG. 3 Lectin-binding pattern of S. retivirgula extract. Lanes 1 to 9, Lectin names and their specificity. Lane 1, Ricinus communis agglutinin, α-D+β-D-galactose; lane 2, Canavalis ensiformis agglutinin, α-D-mannose, α-D-glucose, α-D-N-acetylglucosamine; lane 3, Galanthus nivalis agglutinin, α-D-mannose; lane 4, Maackia amurensis agglutinin, α-D-N-acetylneuraminic acid; lane 5, Arachis hypogaea agglutinin, β-D-galactose; lane 6, Aleuria aurantia agglutinin, α-L-fucose; lane 7, Tritium vulgaris agglutinin, β-D-N-acetylglucosamine; lane 8, Datura stramonium agglutinin, β-1-4-N-acetylglucosamine, N-acetyl-glucosamine-Ser/Thr; lane 9, Amaranthus caudatus agglutinin, β-D-galactose-1-3-α-D-N-acetylglucosamine-Ser/Thr; lane 10, control. kDA, MW in kilodaltons. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

6 FIG. 4 Dot blot analysis of S. rectivirgula carbohydrate residues produced by hydrazinolysis. IgG2 reactivity of patients’ sera (PS), exposed persons’ sera (EX), and blood donors sera (BD). 5, 10, 15, 25, Micrograms of bacterial extract used for hydrazinolysis. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

7 FIG. 5 IgG2 reactivity of a patients’ pooled sera with two-dimensionally separated modified S. rectivirgula extract after TFMS treatment (A), with non-ConA-binding S. rectivirgula proteins (B), and with ConA-binding S. rectivirgula structures (C). kDa, MW in kilodaltons; 1, antigen Sac r 1; 2, antigen Sac r 2. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

8 FIG. 5 IgG2 reactivity of a patients’ pooled sera with two-dimensionally separated modified S. rectivirgula extract after TFMS treatment (A), with non-ConA-binding S. rectivirgula proteins (B), and with ConA-binding S. rectivirgula structures (C). kDa, MW in kilodaltons; 1, antigen Sac r 1; 2, antigen Sac r 2. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

9 FIG. 5 IgG2 reactivity of a patients’ pooled sera with two-dimensionally separated modified S. rectivirgula extract after TFMS treatment (A), with non-ConA-binding S. rectivirgula proteins (B), and with ConA-binding S. rectivirgula structures (C). kDa, MW in kilodaltons; 1, antigen Sac r 1; 2, antigen Sac r 2. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

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