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Fig. 2 Begomovirus-encoded βC1 proteins interact with plant WRKY20 proteins for differentiated resistance against herbivores. Begomovirus-encoded βC1 proteins.

Published byMarianna Marshall Modified over 5 years ago

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Presentation on theme: "Fig. 2 Begomovirus-encoded βC1 proteins interact with plant WRKY20 proteins for differentiated resistance against herbivores. Begomovirus-encoded βC1 proteins."— Presentation transcript:

1 Fig. 2 Begomovirus-encoded βC1 proteins interact with plant WRKY20 proteins for differentiated resistance against herbivores. Begomovirus-encoded βC1 proteins interact with plant WRKY20 proteins for differentiated resistance against herbivores. (A) Interaction of CLCuMuV βC1 (βC1-C) with GhWRKY20 homologs (GhWRKY20-1 and GhWRKY20-2) and TYLCCNV βC1 with AtWRKY20 in a yeast two-hybrid system. (B) BiFC analysis of βC1 proteins interaction with WRKY20 homologous proteins. Scale bars, 50 μm. (C and D) Daily number of eggs laid per female whitefly on vector control cotton and VIGS cotton silenced for GhWRKY20 (C) or on Arabidopsis Col-0 and wrky20 mutant plants (D). (E) Pupa numbers of whiteflies present on Arabidopsis Col-0 and wrky20 mutant plants. (F) Daily number of eggs laid per female whitefly on Arabidopsis Col-0 and overexpressing AtWRKY20 plants. (G to I) Larval weight of CBM reared on vector control cotton and GhWRKY20 VIGS cotton (G) on Col-0 and wrky20 mutant plants (H) or on Col-0 and plants overexpressing AtWRKY20 (I). (C to F) Bars represent means ± SD (n = 8). Means with different letters (a, b, and c) are significantly different [P < 0.05, one-way analysis of variance (ANOVA) along with Duncan’s multiple range test]. (G to I) Bars represent means ± SD (n = 10) (*P < 0.05 and **P < 0.01, Student’s t test). Pingzhi Zhao et al. Sci Adv 2019;5:eaav9801 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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