1. Pinene is produced in malaria parasites by de novo isoprenoid biosynthesis.
Pinene is produced in malaria parasites by de novo isoprenoid biosynthesis. (A to D) Extracted ion chromatograms (EIC) of the total ion chromatograms (TIC) at m/z 93, the base peak in the ion spectrum of pinene. (A) α-(+)-Pinene standard (positive control); (B) uninfected RBCs (negative control); (C) Plasmodium-infected RBCs; (D) Plasmodium-infected RBCs treated with 5 µM fosmidomycin (inhibitor of parasite isoprenoid biosynthesis). (E) EI mass spectra of the observed pinene peak from P. falciparum RBCs in panel C (top) compared to the purified α-(+)-pinene standard in panel A (bottom).
Megan Kelly et al. mBio 2015; doi: /mBio