1. ASSESSMENT OF METAL CONTENT IN SERUM OF DYALIZED PATIENTS
63rd ICASS
Sara Valderrama,a Ricardo Molina,a Carlos del Pozo,a José-Luis Todolíb
a Hospital Virgen de los Lirios, Calle Caramanxel, s/n, 03804, Alcoy, Alicante, Spain.
b Department of Analytical Chemistry, Nutrition and Food Science. University of Alicante. P.O. Box 99, E Alicante, Spain
Hospital Virgen de los Lirios, Alcoy
INTRODUCTION
EXPERIMENTAL
Sample collection & preparation
Metals and metalloids
Hemodialysis
→ Validation of a ICP-MS methodology for serum analysis
→ Quantification of fourteen elements in 1:10 diluted serum
→ Elemental analysis for the control population
→ Preliminary studies for major (Na, K,Ca, Mg) and additional elements (Br, Sn, Nb).
1:10
ICP-MS analysis
METHOD VALIDATION Co Cr Ni
Precision:
5 – 10 % RSD
Accuracy:
Most of the elements
within ± 20% Mo As Sb Zn Rb Se
Comparison of the normalized intensity dialyzed patients (blue bars) versus the normalized intensity for control population (red line) Mn V Cu Sr Ba
Percentage of patients with elemental content above, below and similar to that for the control population as a function of the element
118Sn
CONCLUSIONS
ICP-MS provides accurate results in the trace analysis of human serum through internal standardization.
Cr, Co, Ni, As, Mo and Sb accumulate in hemodialyzed patitens whereas Zn, Se and Rb are depleted with respect to a control population. No differences between groups exist for elements such as V, Mn, Cu, Sr and Ba
Additional differences in elemental content can be found for Si, Nb, Br, Sn (K and Mg).