1. Colonic Treg TCRs react to MA Helicobacter species.
Colonic Treg TCRs react to MA Helicobacter species. (A) Treg TCRs preferentially react to MA antigens (Ags). Hybridoma cells expressing different TCRs were cultured with CD11c+ DCs, and the indicated antigen was obtained 2 weeks after the initiation of colitis. NFAT (nuclear factor of activated T cells)–GFP up-regulation was assessed by flow cytometry 1.5 days later. (B) Selective elimination of MA antigen using individual antibiotics. TCRs from (A) that react to MA antigen were stimulated with colonic MA antigens isolated from antibiotic-treated or untreated mice as per (A). (C) Changes in H. typhlonius and H. apodemus OTUs correlate with in vitro reactivity to MA antigen in (B). Data are the percentage of 16S OTUs from the MA preparations of individual antibiotic-treated or untreated mice. (D) In vitro recognition of H. typhlonius or H. apodemus. Cultured isolates were tested for TCR reactivity in vitro as per (A). Two to three independent experiments. Bars indicate mean.
Jiani N. Chai et al. Sci. Immunol. 2017;2:eaal5068
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