1. Schematic representation of single-ended mcr-1 cassettes that have retained the last 42 bp (A) or 90 bp (B) of an ancestral ISApl1 that includes the entire IRR. The conserved, ancestral CG dinucleotide on the IE of the downstream ISApl1 is indicated with a black triangle.
Schematic representation of single-ended mcr-1 cassettes that have retained the last 42 bp (A) or 90 bp (B) of an ancestral ISApl1 that includes the entire IRR. The conserved, ancestral CG dinucleotide on the IE of the downstream ISApl1 is indicated with a black triangle. The bases upstream and downstream of the deletion that are retained after ISApl1 loss are highlighted in red and blue, respectively. The deletion joints upstream and downstream of the ISApl1 are encased in a black rectangle. The bases upstream and downstream of the deletion that are retained after ISApl1 loss are highlighted in red and blue, respectively, while the remaining copy of the deletion joint that is retained after the two ends are joined following ISApl1 excision is highlighted in green and encased in a black rectangle. The same labeling scheme is used throughout the figures. Putative 2-bp TSDs in 5 of the 7 sequences are highlighted in bold.
Erik Snesrud et al. mBio 2018; doi: /mBio