1. Accumulation of the lipid A pathway intermediate UDP-3-O-[(R)-3-OH-C14]-GlcNAc in the fabZ (FabZR121H) mutant JWM0009 determined by LC-MS/MS.
Accumulation of the lipid A pathway intermediate UDP-3-O-[(R)-3-OH-C14]-GlcNAc in the fabZ (FabZR121H) mutant JWM0009 determined by LC-MS/MS. The increase of β-hydroxymyristoyl-ACP, a precursor molecule for lipid A biosynthesis (Fig. 1), was observed in the fabZ mutant, and this finding suggests that FabZR121H is redirecting intermediate flux into the lipid A biosynthetic pathway. As expected, UDP-3-O-[(R)-3-OH-C14]-GlcNAc did not accumulate in the lpxC mutant JWM0010 (data not shown). Data were normalized to an internal standard (IS) and the culture OD600. Each experiment was averaged from eight replicates.
Mina Mostafavi et al. mSphere 2018; doi: /mSphere