1. Biological effects of BRAF silencing: growth curves of A375 (A) and ARO (B) cell lines in the absence or presence of doxycycline.
Biological effects of BRAF silencing: growth curves of A375 (A) and ARO (B) cell lines in the absence or presence of doxycycline. Cells were counted by trypan blue exclusion assay. C and D. Cell cycle analysis of A375 (C) and ARO (D) cells after 96 h of shRNA induction. An increase in sub-G1 fraction is evident in A375 cells, whereas the ARO cell line showed a decrease of the G2-M peak. E and F. Annexin V staining of A375 (E) and ARO (F) cells was assayed before (time 0) and 48 to 96 h after vehicle or doxycycline treatment. White columns, control cells; black columns, doxycycline-treated cells; the average of triplicate samples is always reported, with SD (*, P < 0.01; **, P < 0.001). G. ARO cells were treated with vehicle alone (−) or doxycycline (+) for 9 d; induction of NIS expression is shown by Western blot. Actin was used as a loading control.
Elisa Sala et al. Mol Cancer Res 2008;6:
©2008 by American Association for Cancer Research