1. Manipulation of Sox11 shifts the abundance of differentiated neurons and apical progenitors.
Manipulation of Sox11 shifts the abundance of differentiated neurons and apical progenitors. A–C, At E14.5, both Tbr1+ (A) and Ctip2+ (B) cells are reduced in Sox11CKO cortex compared with control. D, E, In utero electroporation that caused either elevation or reduction of Sox11 resulted in more or fewer transfected cells, respectively, becoming GFP+dsRed+ neurons. F–H, At E14.5, Sox2+ (F) and Sox9+ (G) cell numbers are elevated in Sox11CKO cortex compared with control. I, J, In utero electroporation that caused either elevation or reduction of Sox11 resulted in no significant change for GOF and more transfected cells staining for the apical progenitor marker Sox9 for LOF. K, IHC for Tbr2 revealed no change in the number of cortical cells expressing the protein in Sox11CKO mice compared with control (control: 6864 ± 140/mm2, Sox11 CKO: 6888 ± 587/mm2, p = 0.96). L–N, At E12.5 (L) and P0 (M), there was no change in activated Caspase3 staining in Sox11CKO cortex compared with control mice. Scale bars: A, B, F, G, K, 24 μm; D, 20 μm; I, 17 μm; L, 32 μm; M, 75 μm.
Chao Chen et al. J. Neurosci. 2015;35:
©2015 by Society for Neuroscience