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Fig. 3 Prophylactic or therapeutic use of DECON protects from herpes infections in vitro. Prophylactic or therapeutic use of DECON protects from herpes.

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Presentation on theme: "Fig. 3 Prophylactic or therapeutic use of DECON protects from herpes infections in vitro. Prophylactic or therapeutic use of DECON protects from herpes."— Presentation transcript:

1 Fig. 3 Prophylactic or therapeutic use of DECON protects from herpes infections in vitro.
Prophylactic or therapeutic use of DECON protects from herpes infections in vitro. (A) Fluorescent images showing extent of HSV-1 infection (green) in HCEs treated with either ACV-loaded DECON, DMSO-loaded DECON, HPAC alone, mock DMSO, prophylactically added ACV, or therapeutically added ACV. Scale bar (similar for all images), 100 μm. (B) Flow cytometry conducted on the samples at 24 hpi showing the extent of cells infected with HSV-1 GFP. Cells to right of dashed line show fluorescence exceeding that of negative controls. (C) Representative immunoblots (top) and quantification (bottom) for samples showing HSV-1 gB protein in comparison with GAPDH for HSV-1–infected HCEs at 24 hpi. (D to F) Plaque assay data showing extent of virus inhibition by DECON in comparison to ACV, HPAC, and mock DMSO for HSV-2, PRV, and BHV for intracellular virus collected 24 hpi. Data are presented as means ± SD. Significance to mock-treated cells was determined by one-way ANOVA followed by Dunnett’s multiple comparisons test (n = 3 replicates). Tejabhiram Yadavalli et al. Sci Adv 2019;5:eaax0780 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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