1. Immune evasion occurs independently of perforin-mediated killing.
Immune evasion occurs independently of perforin-mediated killing. (A) MC38Ova/Vector chromium release assay (18 hours) at the indicated E:T ratios with Prf1−/− OT-I T cells. B16Ova/Vector coculture (48 hours) with Prf1−/− OT-I T cells (2:1 E:T ratio). DAPI, 4′,6-diamidino-2-phenylindole. (B) MC38Ova screen as in Fig. 1A, but using Prf1−/− OT-I T cells. (C) B16Ova screen, as in Fig. 1J, but using Prf1−/− OT-I T cells. (D) Comparison of top enriched genes in screens described in (B) and (C). (E) Selected sgRNA counts from (B) and (C). (F) Protein network analysis of the top scoring genes from (B). (G) GO term analysis from the top scoring genes from (B). (H) Comparison of top enriched genes in screens described in Figs. 1C and 2C. KO, knockout. (I) Protein network analysis of the top scoring genes from (B). (J) GO term analysis of the top scoring genes from (C). (K) Comparison of top enriched genes in screens described in Fig. 1J and (C).
Conor J. Kearney et al. Sci. Immunol. 2018;3:eaar3451
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