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Evidence for a lipid transfer protein as the major allergen of apricot

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Presentation on theme: "Evidence for a lipid transfer protein as the major allergen of apricot"— Presentation transcript:

1 Evidence for a lipid transfer protein as the major allergen of apricot
Elide A. Pastorello, MDa, Francesco Purello D’Ambrosio, MDb, Valerio Pravettoni, MDa, Laura Farioli, BScc, Gabriella Giuffrida, BScd, Mara Monza, BSca, Raffaella Ansaloni, MDe, Donatella Fortunato, BScd, Elisabetta Scibola, MDa, Federica Rivolta, MDa, Cristoforo Incorvaia, MDa, Anders Bengtsson, BScf, Amedeo Conti, BScd, Claudio Ortolani, MDe  Journal of Allergy and Clinical Immunology  Volume 105, Issue 2, Pages (February 2000) DOI: /S (00) Copyright © 2000 Mosby, Inc. Terms and Conditions

2 Fig. 1 IgE immunoblots of apricot extract in 30 patients with OAS. Patients 1 to 16 had birch pollinosis, and patients 17 to 30 did not have birch pollinosis. Journal of Allergy and Clinical Immunology  , DOI: ( /S (00) ) Copyright © 2000 Mosby, Inc. Terms and Conditions

3 Fig. 2 Cation exchange of apricot extract fractionated over the Resource-S HPLC column (A) . Gel filtration of the peak obtained in cation-exchange chromatography (B) . Journal of Allergy and Clinical Immunology  , DOI: ( /S (00) ) Copyright © 2000 Mosby, Inc. Terms and Conditions

4 Fig. 3 SDS-PAGE of total apricot extract and the 9-kd purified protein stained with Coomassie Brilliant Blue and IgE immunoblotting of the 9-kd purified protein. Journal of Allergy and Clinical Immunology  , DOI: ( /S (00) ) Copyright © 2000 Mosby, Inc. Terms and Conditions

5 Fig. 4 Blotting inhibition of crude apricot extract by the peach 9-kd purified protein. Journal of Allergy and Clinical Immunology  , DOI: ( /S (00) ) Copyright © 2000 Mosby, Inc. Terms and Conditions

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