1. ADU-S100–mediated tumor clearance is dependent on CD4+, CD8+ T cells, and NK cells.
ADU-S100–mediated tumor clearance is dependent on CD4+, CD8+ T cells, and NK cells. Tumor-bearing FVB/N mice were depleted of CD4+, CD8+, both CD4+ and CD8+ T cells, and NK cells using biweekly intraperitoneal injections of 250 μg of anti-CD4 (clone GK1.1), anti-CD8 (clone 2.43), or both anti-CD4 and CD8, and anti-NK1.1 (clone PK136). Injection of depleting antibodies began 1 week prior to tumor implantation and depletion efficacy was confirmed by flow cytometry (>90% depletion). Tumor growth (A) and survival (B) after CD4+ T-cell depletion, CD8+ T-cell depletion, CD4+ and CD8+ T-cell depletion, and NK cell depletion (C and D) were measured at the indicated time points. All data are representative of three independent experiments with 5 mice/group. *, P < 0.05; ****, P <
Jeremy B. Foote et al. Cancer Immunol Res 2017;5:
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