1. Expression pattern of TLR-4 and MyD88 in EOC cells.
Expression pattern of TLR-4 and MyD88 in EOC cells. A, evaluation of TLR-4 mRNA expression by reverse transcription-PCR from OSE and EOC cell lines. B and C, Western blot analysis for TLR-4 and MyD88 protein expression from EOC cell lines and ovarian cancer tumor samples. +, positive control; −, negative control (no reverse transcription). D, laser capture microdissection of ovarian cancer cells from tissue sections before (Pre) and after dissection (Post). E, Western blot analysis for the expression of TLR-4 and MyD88 by ovarian cancer cells following laser microdissection. Lane 1, positive control (macrophages); lane 2, 6,000 EOC cells microdissected by laser. Representative of 10 tumors evaluated.
Michael G. Kelly et al. Cancer Res 2006;66:
©2006 by American Association for Cancer Research