1. Production of a recombinant imported fire ant venom allergen, Sol i 2, in native and immunoreactive form 
Margit Schmidt, PhDa, b, Thomas J. McConnell, PhDc, Donald R. Hoffman, PhDb 
Journal of Allergy and Clinical Immunology 
Volume 98, Issue 1, Pages (July 1996)
DOI: /S (96)
Copyright © 1996 Mosby, Inc. Terms and Conditions

2. FIG. 1
Isolation of rSol i 2 from concentrated diafiltered supernatant of recombinant baculovirus grown in High-Five cells in Ex-Cell 400 medium on a Super-ODS reverse-phase column at 50° C with a gradient of acetonitrile in water containing TFA as an ion-pairing agent. The fraction from to minutes was collected, corresponding to the elution position of nSol i 2.
Journal of Allergy and Clinical Immunology  , 82-88DOI: ( /S (96) )
Copyright © 1996 Mosby, Inc. Terms and Conditions

3. FIG. 2
Silver-stained SDS-PAGE gel of culture supernatants from Sol i 2 recombinant baculovirus grown in Sf9 cells. Lanes from left to right are: n, natural Sol i 2; G, rSol i 2 baculovirus grown in Grace’s medium; Gm, Grace’s medium without serum; Mc, Sf9 cells grown in Sf-900 II SFM; 2, 10, and 13, clones 2, 10, and 13 of rSol i 2 baculovirus in Sf9 cells grown in Sf-900 II SFM.
Journal of Allergy and Clinical Immunology  , 82-88DOI: ( /S (96) )
Copyright © 1996 Mosby, Inc. Terms and Conditions

4. FIG. 3
Dot immunoblots of natural Sol i 2 and rSol i 2 with monoclonal antibodies G260 and A4. Lanes 1, 2, and 3 are natural Sol i 2; lanes 4, 5, and 6 are rSol i 2. Dots 1 and 4 are untreated, dots 2 and 5 are boiled in SDS, and dots 3 and 6 are boiled in SDS in the presence of dithiothreitol.
Journal of Allergy and Clinical Immunology  , 82-88DOI: ( /S (96) )
Copyright © 1996 Mosby, Inc. Terms and Conditions

5. FIG. 4
A, Silver-stained SDS-PAGE gel of culture supernatants from two flasks of recombinant baculovirus grown in High-Five cells. Lanes are labeled n for natural Sol i 2; 1,2 for supernatants from rSol i 2 baculovirus; and c for control cells grown in Ex-Cell 400 medium. B, Silver-stained SDS-PAGE gel of purified natural (n) and recombinant (r) Sol i 2, each at three different concentrations.
Journal of Allergy and Clinical Immunology  , 82-88DOI: ( /S (96) )
Copyright © 1996 Mosby, Inc. Terms and Conditions

6. FIG. 4
A, Silver-stained SDS-PAGE gel of culture supernatants from two flasks of recombinant baculovirus grown in High-Five cells. Lanes are labeled n for natural Sol i 2; 1,2 for supernatants from rSol i 2 baculovirus; and c for control cells grown in Ex-Cell 400 medium. B, Silver-stained SDS-PAGE gel of purified natural (n) and recombinant (r) Sol i 2, each at three different concentrations.
Journal of Allergy and Clinical Immunology  , 82-88DOI: ( /S (96) )
Copyright © 1996 Mosby, Inc. Terms and Conditions

7. FIG. 5
Plot of RAST specific binding of sera from 24 patients allergic to imported fire ant venom to natural Sol i 2 and rSol i 2. Linear regression coefficient is r = 0.94, with a slope of 1.08.
Journal of Allergy and Clinical Immunology  , 82-88DOI: ( /S (96) )
Copyright © 1996 Mosby, Inc. Terms and Conditions