1. PHF10 acts as a transcriptional repressor.
PHF10 acts as a transcriptional repressor. A, top, localization of PHF10 in gastric cancer cells. A coverslip seeded with SGC7901 cells was double stained with PHF10 antibody to permit detection of PHF10 (a) and phalloidin to permit detection of actin (b). A DNA-binding dye, DAPI, was used to counterstain the DNA (c). In SGC7901s cells, PHF10 accumulated in nuclear domains that colocalize with dense regions of DAPI staining (d), indicating association with chromatin. Middle, histogram showing transcriptional activity analysis of PHF10 fusion proteins in 293T cells. Columns, mean of three experiments; bars, SD. *, P < 0.05 versus control, cotransfection of pCMV-BD-V and pLexA-VP16. Diagram at the bottom depicts the mechanisms underlying this assay. B, transcriptional activity analysis of full-length and truncated PHF10 fusion proteins in 293T cells. Columns, mean of three experiments; bars, SD. *, P < 0.05 versus cotransfection of pCMV-BD-V and pL8G5 luciferase, which was used as control.
Min Wei et al. Mol Cancer Ther 2010;9:
©2010 by American Association for Cancer Research