1. Lipid-mediated ex vivo gene transfer of viral interleukin 10 in rat lung allotransplantation 
Hideki Itano, MDa, Bassem N. Mora, MDa, Wanjiang Zhang, MDa, Jon H. Ritter, MDb, Timothy J. McCarthy, PhDc, Nelson S. Yew, PhDd, T. Mohanakumar, PhDa, G.Alexander Patterson, MDa 
The Journal of Thoracic and Cardiovascular Surgery 
Volume 122, Issue 1, Pages (July 2001)
DOI: /mtc
Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

2. Fig. 1
Semiquantitative RT-PCR analysis of vIL-10 mRNA transgene expression in rat lung allografts. The peak areas of bands were expressed as a relative ratio of vIL-10/glyceraldehyde-3-phosphate dehydrogenase (GA3PDH). The gel image shows vIL-10 bands from groups 1 and 2 but not from group 3. The relative ratio of vIL-10/glyceraldehyde-3-phosphate dehydrogenase in group 1 was significantly higher than that in group 2 (P =.0050 vs group 2). Values are means ± SD of 6 or 7 animals per group. PC, Positive control.
The Journal of Thoracic and Cardiovascular Surgery  , 29-38DOI: ( /mtc )
Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

3. Fig. 2
Immunohistochemistry for transgene vIL-10. Transgene vIL-10 protein was detected by means of immuno-histochemistry in bronchial endothelial cells, alveolar epithelial cells, and alveolar macrophages. (Original magnifications: A, 200×; B, 200×; C, 400×; D, 400×.)
The Journal of Thoracic and Cardiovascular Surgery  , 29-38DOI: ( /mtc )
Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

4. Fig. 3
Histologic rejection score. The sections were graded separately for both acute vascular rejection (A-score) and airway rejection (B-score). The acute vascular rejection score in group 1 was significantly lower than that in group 3 (P =.042 vs group 3, Kruskal-Wallis test). There were no significant differences (NS) in airway rejection scores. Bars show median values.
The Journal of Thoracic and Cardiovascular Surgery  , 29-38DOI: ( /mtc )
Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

5. Fig. 4
Arterial blood oxygen tension (PaO2). PaO2 obtained 5 minutes after right hilar crossclamping is shown. Each PaO2 in groups 1 ( ± mm Hg) and 2 ( ± 35.7 mm Hg) was significantly higher than that in group 3 (66.4 ± 8.22 mm Hg; P =.020 and P =.023 vs group 3). Values are means ± SD of 6 or 7 animals per group.
The Journal of Thoracic and Cardiovascular Surgery  , 29-38DOI: ( /mtc )
Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

6. Fig. 5
Exhaled NO levels from rat lung allografts. Exhaled NO levels in group 2 (5.150 ± 6.38 ppb) were significantly lower than those in group 3 ( ± 10.4 ppb; P =.020 vs group 3). Exhaled NO levels in normal lungs (NL ; 0.00 ± 0.0 ppb) were significantly lower than those in groups 1 and 3 (*P <.005, respectively). Values are means ± SD of 5 to 7 animals per group.
The Journal of Thoracic and Cardiovascular Surgery  , 29-38DOI: ( /mtc )
Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

7. Fig. 6
Semiquantitative RT-PCR analysis of IL-2 mRNA expression in rat lung allografts. The peak areas of the bands were expressed as the relative ratio of IL-2/glyceraldehyde-3-phosphate dehydrogenase (GA3PDH). IL-2 mRNA expression in group 1 and in normal lungs was significantly lower than that in group 3 (P =.039 and P =.0014 vs group 3). IL-2 mRNA expression in group 2 was lower than that in group 3, trending toward significance (P =.068 vs group 3). Values are means ± SD of 6 or 7 animals per group.
The Journal of Thoracic and Cardiovascular Surgery  , 29-38DOI: ( /mtc )
Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions

8. Fig. 7
RT-PCR analysis for mRNA expression of tumor necrosis factor (TNF) α, iNOS, IFN-γ, and endogenous IL-10 in rat lung allografts. Normal lung (NL) mRNA expression in these cytokines was significantly lower than that in other groups (*P <.05, respectively). There were no significant differences between groups 1, 2, and 3. Values are means ± SD of 4 to 7 animals per group. GA3PDH, Glyceraldehyde-3-phosphate dehydrogenase.
The Journal of Thoracic and Cardiovascular Surgery  , 29-38DOI: ( /mtc )
Copyright © 2001 American Association for Thoracic Surgery Terms and Conditions