1. RNase protection assay illustrating multiple mRNAs elicited by microglia in response to Acanthamoeba.
RNase protection assay illustrating multiple mRNAs elicited by microglia in response to Acanthamoeba. Primary microglial cells obtained from brain cerebral cortices of newborn rat were cocultured with A. castellanii, A. culbertsoni, or bacterial lipopolysaccharide (LPS) for 6 h. The cultures then were harvested with Trizol, and the RNA was subjected to RNase protection assay analysis for assessment of inducible cytokine gene expression. Microglial cells alone (lane MIC), microglia incubated with LPS (lane MIC + LPS), microglia incubated with A. culbertsoni (lane MIC + Ac), and microglia incubated with A. castellanii (lane MIC + Ac50494) are shown. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Francine Marciano-Cabral, and Guy Cabral Clin. Microbiol. Rev. 2003; doi: /CMR