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Flow cytometry analysis of intracellular cytokine expression in control PBMC (left set of panels) and following either PMA-ionomycin activation (middle.

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Presentation on theme: "Flow cytometry analysis of intracellular cytokine expression in control PBMC (left set of panels) and following either PMA-ionomycin activation (middle."— Presentation transcript:

1 Flow cytometry analysis of intracellular cytokine expression in control PBMC (left set of panels) and following either PMA-ionomycin activation (middle set of panels) or PHA activation (right set of panels). Flow cytometry analysis of intracellular cytokine expression in control PBMC (left set of panels) and following either PMA-ionomycin activation (middle set of panels) or PHA activation (right set of panels). PBMCs were cultured in medium alone or were stimulated with PMA-ionomycin or PHA in the presence of monensin for 6 h and stained with PE-labeled anti-cytokine MAbs. Histogram overlays show the FL2 (orange fluorescence) intensity corresponding to a given cytokine (solid line) compared to the intensity for the isotype-specific control (dotted line). The numbers indicate the percentages of positive cells and the mean fluorescence intensity (mfi). The results from one representative experiment of five experiments performed are shown. Jarołsaw Baran et al. Clin. Diagn. Lab. Immunol. 2001; doi: /CDLI


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