1. CLIC1 is upregulated in invadopodia of fibrin-embedded tumor and endothelial cells.
CLIC1 is upregulated in invadopodia of fibrin-embedded tumor and endothelial cells. A, confocal microscopy images of HUVEC or cells embedded in fibrin clots for 1 or 2 hours after immunostaining with anti-CLIC1 (red). Nuclei are stained with draq5 (blue). B, confocal microscopy images of 24-hour fibrin-embedded HUVEC and cells after immunostaining for β3 integrin (green) and CLIC1 (red). Nuclei are stained with draq5 (blue). C, fibrin-embedded cells transformed with scrambled (shSCR, top) or β3 shRNA (shβ3, bottom) were fixed after indicated times and probed for CLIC1 (green) and F-actin (red). Nuclei are stained with draq5 (blue). Scale bars, 10 μm.
Lisa A. Gurski et al. Mol Cancer Res 2015;13:
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