Presentation is loading. Please wait.

Presentation is loading. Please wait.

Remodeling of experimental arteriovenous fistula with increased matrix metalloproteinase expression in rats  Chih-Yang Chan, MD, Yih-Sharng Chen, MD,

Published byสมศักดิ์ รักไทย Modified over 5 years ago

Similar presentations

Presentation on theme: "Remodeling of experimental arteriovenous fistula with increased matrix metalloproteinase expression in rats  Chih-Yang Chan, MD, Yih-Sharng Chen, MD,"— Presentation transcript:

1 Remodeling of experimental arteriovenous fistula with increased matrix metalloproteinase expression in rats  Chih-Yang Chan, MD, Yih-Sharng Chen, MD, PhD, Ming-Chieh Ma, PhD, Chou-Fong Chen, PhD  Journal of Vascular Surgery  Volume 45, Issue 4, Pages (April 2007) DOI: /j.jvs Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 Schematic diagram of the arteriovenous fistula model. (a) After an incision was made in the left groin, the femoral artery (a) and vein (v) were carefully dissected. (b) The proximal ends of both vessels close to the abdomen were clamped and the distal ends ligated using 4-0 silk. (c) The distal end of the femoral artery was cut and a venotomy was created using iris scissors under a dissecting microscopy. (d) The anastomosis of the femoral artery and vein was constructed by invagination of the cut end of the artery into the vein. (e) Ligature using 8-0 silk was performed to prevent the artery from detaching after reflow. (f) The clamp on the proximal end of the artery was then released and the blood in the vein quickly turned bright red and the vein expanded. (g) The groin was then closed under aseptic conditions. (h) The vascular structures for the left arteriovenous shunt are shown. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Changes in the thickness of the layers in the iliac vein. A, Temporal changes in the mean thickness of the intima and media and the intima/media thickness ratio of the left iliac vein in the sham and fistula groups. *P < .05 compared with the corresponding sham group. B, Representative vessel segments of the right and left iliac veins from sham and fistula rats at day 14 or 28. Note that the vessel walls on days 14 and 28 are thicker than at the same time point on either the contralateral right side in the same animal or on the same side in sham rats. Reduced from original magnification ×400. The horizontal bar indicates 20 μm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 Upregulation of matrix metalloproteinase (MMP) 2 and 9. A, Changes in the expressions of MMP2 and B, MMP9 in six rats examined by Western blotting. The bar graphs show that the change to MMP2 or MMP9/actin density unit (DU) ratio. *P < .05 compared with the sham group (days 14 and 28 pooled). C, Linear regression shows that the amounts of MMP2 and 9 are positively correlated with the thickness of the intima. D, Representative micrographs show the location of MMP2 and MMP9 in situ by immunohistochemical staining at 28 days in sham rat and a fistula rat. Reduced from original magnification ×400. The black arrows and horizontal bar indicate positive signals and 100 μm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 A, Decreased levels of tissue inhibitor of matrix metalloproteinase-4 and B, collagen I/III. Representative blots from six (TIMP-4) and four (collagen I and III) rats. The bar graphs show that the change to TIMP-4/actin density unit (DU) ratio and collagen I or III/actin DU ratio. *P < .05 compared with the sham group (pooled day 14 and 28 samples). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

Download ppt "Remodeling of experimental arteriovenous fistula with increased matrix metalloproteinase expression in rats  Chih-Yang Chan, MD, Yih-Sharng Chen, MD,"

Similar presentations

Grafting an Acellular 3-Dimensional Collagen Scaffold Onto a Non-transmural Infarcted Myocardium Induces Neo-angiogenesis and Reduces Cardiac Remodeling 

Grafting an Acellular 3-Dimensional Collagen Scaffold Onto a Non-transmural Infarcted Myocardium Induces Neo-angiogenesis and Reduces Cardiac Remodeling 
Vascular changes at the puncture segments of arteriovenous fistula for hemodialysis access  Ju-Feng Hsiao, MD, Hsin-Hua Chou, MD, Lung-An Hsu, MD, PhD,

Vascular changes at the puncture segments of arteriovenous fistula for hemodialysis access  Ju-Feng Hsiao, MD, Hsin-Hua Chou, MD, Lung-An Hsu, MD, PhD,
Diet-induced obesity drives negative mouse vein graft wall remodeling

Diet-induced obesity drives negative mouse vein graft wall remodeling
Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.
Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD 

Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD 
Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis  April E. Nedeau, MD,

Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis  April E. Nedeau, MD,
Volume 85, Issue 2, Pages (January 2014)

Volume 85, Issue 2, Pages (January 2014)
Chronic kidney disease aggravates arteriovenous fistula damage in rats

Chronic kidney disease aggravates arteriovenous fistula damage in rats
Coarctation-induced degenerative abdominal aortic aneurysm in a porcine model  Pao-Yen Lin, MD, Yeng-Ting Wu, MS, Guan-Cheng Lin, BS, Yao Hsiang Shih,

Coarctation-induced degenerative abdominal aortic aneurysm in a porcine model  Pao-Yen Lin, MD, Yeng-Ting Wu, MS, Guan-Cheng Lin, BS, Yao Hsiang Shih,
Chun-Yu Wong, MD, Margreet R. de Vries, BSc, Yang Wang, MD, Joost R

Chun-Yu Wong, MD, Margreet R. de Vries, BSc, Yang Wang, MD, Joost R
Large external iliac vein aneurysm in a patient with a post-traumatic femoral arteriovenous fistula  Peter J. Kuhlencordt, MD, Ulrich Linsenmeyer, MD,

Large external iliac vein aneurysm in a patient with a post-traumatic femoral arteriovenous fistula  Peter J. Kuhlencordt, MD, Ulrich Linsenmeyer, MD,
VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery  Yanming Qu, MD, Xiangen Shi, MD, Hongwei Zhang,

VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery  Yanming Qu, MD, Xiangen Shi, MD, Hongwei Zhang,
Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.
Loss of STAT1 is associated with increased aortic rupture in an experimental model of aortic dissection and aneurysm formation  Matthew J. Eagleton, MD,

Loss of STAT1 is associated with increased aortic rupture in an experimental model of aortic dissection and aneurysm formation  Matthew J. Eagleton, MD,
Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.

Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.
Hernan A. Bazan, MD, Harry Schanzer, MD  Journal of Vascular Surgery 

Hernan A. Bazan, MD, Harry Schanzer, MD  Journal of Vascular Surgery 
Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.
Lipoplex gene transfer of inducible nitric oxide synthase inhibits the reactive intimal hyperplasia after expanded polytetrafluoroethylene bypass grafting 

Lipoplex gene transfer of inducible nitric oxide synthase inhibits the reactive intimal hyperplasia after expanded polytetrafluoroethylene bypass grafting 
Matrix metalloproteinase inhibition reduces intimal hyperplasia in a porcine arteriovenous-graft model  Joris I Rotmans, MD, Evelyn Velema, BSc, Hence.

Matrix metalloproteinase inhibition reduces intimal hyperplasia in a porcine arteriovenous-graft model  Joris I Rotmans, MD, Evelyn Velema, BSc, Hence.
Creation and closure of temporary arteriovenous fistulas for venous reconstruction or thrombectomy: Description of technique  Richard J. Sanders, M.D.,

Creation and closure of temporary arteriovenous fistulas for venous reconstruction or thrombectomy: Description of technique  Richard J. Sanders, M.D.,

Similar presentations

Ads by Google