1. CDCP1 links Ras and SFK signaling and regulates anoikis resistance, cell migration, and invasion in NSCLC cells.
CDCP1 links Ras and SFK signaling and regulates anoikis resistance, cell migration, and invasion in NSCLC cells. A, A549, H322, and H322Fyn cells treated with H-Ras-12V and/or CDCP1 siRNA were analyzed by immunoblotting. B, the effect of H-Ras-12V on anchorage independence was determined by soft-agar assay. C, the effect of H-Ras-12V on cell death in suspension culture was determined by Trypan blue staining as a percentage. D, cell proliferation in suspension culture of each cells were determined with a cell proliferation ELISA BrdUrd Kit. E, A549 cells that stably expressed CDCP1 tagged with FLAG (CDCP1-F), a CDCP1 mutant tagged with FLAG (Y734F-F), and control pcDNA3.1 plasmid (pcDNA) were treated with PD98059 for 24 hours and analyzed by immunoblotting. F, the effect of CDCP1-F or Y734F-F on anchorage independence in A549 cells were determined by soft-agar assay. G, rescue of cell migration by phosphorylated CDCP1. H, rescue of cell invasion by phosphorylated CDCP1. Black arrowheads indicate phosphorylated CDCP1. White arrowheads indicate cross-reactive bands. Each data are the means ± SD from three experiments. *, P values in a Student t test < Black arrowheads denote band corresponding to CDCP1. White arrowhead indicates nonspecific bands.
Takamasa Uekita et al. Mol Cancer Res 2014;12:
©2014 by American Association for Cancer Research