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Uptake of TEX by DCs in vivo.
Uptake of TEX by DCs in vivo. PKH67-labeled TEX (10 μg) were injected s.c. at the base of the tail, and the DLNs were collected 24, 48, and 72 hours after injection (n = 3/group per time point). DLN cells were stained with anti-CD11c to identify DCs. A, Representative flow cytometry plots gated on CD11c+ DCs. Boxes encase PKH67+ DCs. B, Mean percentage ± SEM of TEX+ CD11c+ cells at different times postinjection. Using a Student two-tailed t test; *, P < 0.05. Julie M. Diamond et al. Cancer Immunol Res 2018;6: ©2018 by American Association for Cancer Research
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