1. A, gel retardation assay in colon cancer cell line SW480.
A, gel retardation assay in colon cancer cell line SW480. A Tcf4-specific, double-stranded oligonucleotide was used as the probe. Arrow, supershift of theβ -catenin/Tcf4 complex after incubation with anti β-catenin monoclonal antibody. B and C, immunocytochemical staining of β-catenin in SW480 cells after 72 h of infection with either adenovirus containing the 20-amino acid repeat region of the wild-type APC gene (Ad-APC) at 100 multiplicity of infection or LacZ (Ad-LacZ). D, expression of DRCTNNB1A in SW480 cells. Cells were untreated or transfected for 72 h with either Ad-LacZ or Ad-APC. Lower panel, Western blot analysis ofβ -catenin in corresponding cells.
Teru Kawasoe et al. Cancer Res 2000;60:
©2000 by American Association for Cancer Research