1. Representative cytokine and cytotoxicity responses to CMV peptides on day 0 and day 7 in one HLA A2+ donor.
Representative cytokine and cytotoxicity responses to CMV peptides on day 0 and day 7 in one HLA A2+ donor. (A) CFC of CD3+ CD8+ lymphocytes from unstimulated PBMC (left panel), or PBMC stimulated with a pp65 HLA A2-restricted-peptide (amino acids 495 to 503, NLVPMVATV) for 6 h on day 0 (middle panel) or 7 days (right panel). (B) Four-hour51Cr release assay of purified CD8+ lymphocytes after 7 days of peptide restimulation in the same donor as in panel A. Target cells were B-LCL or JY cells loaded with HLA A2-restricted peptide, control (ctrl.) peptide (MVATVGGGA), or no peptide. (C) Example of CFC responses to HLA-A2-restricted peptide (left panels) versus HLA B7-restricted peptide (pp65 amino acids 418 to 426, PRVTGGGAM) (right panels) in a second donor who was HLA A2+ B7+, stimulated for 6 h (top panels) or 7 days (lower panels). This donor, although HLA A2+, responded predominantly to the HLA B7-restricted epitope. (D) Four-hour51Cr release assay of purified CD8+ lymphocytes after 7 days of HLA A2-restricted peptide stimulation in the same donor as in panel C. Target cells were B-LCL loaded with HLA A2-restricted or HLA B7-restricted peptide. Results correlated with those obtained by CFC in panel C.
Smita A. Ghanekar et al. Clin. Diagn. Lab. Immunol. 2001; doi: /CDLI