1. TET2 interacts with KLF1 transcription factor.
TET2 interacts with KLF1 transcription factor. The catalytic domain of TET2 was transfected into the UT-7 erythroid cell line and used to determine interaction between TET2 and erythroid-specific transcription factor KLF1. A and B, Reciprocal immunoprecipitations were performed using total cell lysates and performing either immunoprecipitation with an anti-KLF1 antibody followed by immunoblot analysis using an anti-FLAG antibody or immunoprecipitation using an anti-FLAG antibody followed by an anti-KLF1 antibody. Anti-KLF1 or anti-FLAG antibodies were used as controls. C, KLF1 interaction with TET2 catalytic domain is enhanced in response to stimulation with erythropoietin. Anti-FLAG antibody was used as a control to confirm the presence of equivalent amounts of GFP/FLAG-tagged TET2. D–G, Binding affinities between TET2 catalytic protein and KLF1 were determined for each peptide carrying no phosphorylation, Y1939 +Y1964 double phosphorylation, or single residue phosphorylation. p38 MAP kinase was used as a negative control to demonstrate the specificity of the interaction. The data are representative of duplicate determination.
Jong Jin Jeong et al. Cancer Discov 2019;9:
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