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Fig. 2 Isopropanol-tolerant E. faecium resists disinfection.

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Presentation on theme: "Fig. 2 Isopropanol-tolerant E. faecium resists disinfection."— Presentation transcript:

1 Fig. 2 Isopropanol-tolerant E. faecium resists disinfection.
Isopropanol-tolerant E. faecium resists disinfection. A mouse gastrointestinal colonization assay was used to assess transmission of E. faecium. (A) The CD50 (gray dashed lines) for two vancomycin-resistant E. faecium isolates (Ef_aus0004 and Ef_aus0233) and two vancomycin-sensitive isolates (Ef_aus0026 and Ef_aus0099) was established (table S6). (B) The results of the contaminated cage floor mouse gut colonization experiment to quantify transmission of E. faecium are shown. The concentration of E. faecium before (the inoculum) and after standardized cage floor cleaning with 70% (v/v) isopropanol versus cleaning with water is plotted (table S7). The symbols show E. faecium CFU for the floor of three cages. (C) Percentage of mice with gut colonization by vancomycin-resistant or VSE after standardized cage floor cleaning with either 70% (v/v) isopropanol or sterile water. The results of at least three independent experiments based on six mice per experiment are shown (*P < 0.01; table S8). The null hypothesis (no difference between E. faecium that is sensitive versus tolerant to isopropanol) was rejected for P < 0.05, unpaired t test with Welch’s correction. Sacha J. Pidot et al., Sci Transl Med 2018;10:eaar6115 Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works

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