1. Detection of PSA-mRNA by single (first panel, 710 bp) and nested (second panel, 455 bp) RT-PCR.
Detection of PSA-mRNA by single (first panel, 710 bp) and nested (second panel, 455 bp) RT-PCR. Detection of PSMA-mRNA by single (third panel, 401 bp) and nested (fourth panel, 281 bp) RT-PCR. Detection of PSCA-mRNA by single (fifth panel, 340 bp) and nested (sixth panel, 170 bp) RT-PCR. β-actin (beta-actin), a control mRNA (seventh panel, 520 bp). Lane on far left, Molecular weight marker. The next five lanes, LNCaP human PCa cells [0, none (one of the negative controls); 100, 1 cell; 101, 10 cells; 102, 100 cells; 103, 1000 cells] mixed with 1 ml of human female peripheral blood and amplified by RT-PCR. NC., a second negative control, with a reaction containing peripheral blood mononuclear cells cDNA from a healthy young male; a negative control without cDNA was also prepared (data not shown). PC., a positive control.
Noboru Hara et al. Clin Cancer Res 2002;8:
©2002 by American Association for Cancer Research