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Fig. 2 Viperin promotes methionine oxidation of KSHV helicase.

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Presentation on theme: "Fig. 2 Viperin promotes methionine oxidation of KSHV helicase."— Presentation transcript:

1 Fig. 2 Viperin promotes methionine oxidation of KSHV helicase.
Viperin promotes methionine oxidation of KSHV helicase. (A) Mass spectrometry analysis of methionine oxidation in 293T cells transfected with KSHV helicase with or without viperin expression. The M401 was oxidized (in red) under viperin overexpression. m/z, mass/charge ratio. (B) 293T cells were transfected with plasmids containing indicated genes. At 48 hours after transfection, WCLs were harvested and analyzed by immunoblotting. eGFP, enhanced green fluorescent protein. (C) 293T cells were transfected with plasmids containing indicated genes. At 48 hours after transfection, RNA was extracted, and complementary DNA (cDNA) was prepared to determine KSHV helicase mRNA by qPCR analysis. The data are expressed as means ± SEM; n = 3; ns, not significant; CON, control. (D) RGB-FLAG cells were transfected with siRNA as indicated. At 6 hours after transfection, cells were induced with doxycycline (2 μg/ml) for 72 hours. WCLs were then analyzed by immunoblotting. (E) 293T cells were transfected with plasmids containing indicated genes. At 24 hours after transfection, cells were treated with cycloheximide (CHX) (10 μg/ml). WCLs were then analyzed by immunoblotting. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Lei Bai et al. Sci Adv 2019;5:eaax1031 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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